Secondary Development Research Articles

Secondary School State Athletic Association Health and Safety Policy Development Processes.

The National Federation of State High School Associations provides recommendations regarding health and safety policies; however, policy development is governed at the state level. Given interstate differences in governance, the primary purpose was to describe processes that State High School Athletic Associations (SHSAAs) utilize to develop a new policy. The secondary objective was to determine what methods associations use to implement new policies. A cross-sectional survey requested SHSAA (n = 51) representatives to report how athlete health and safety policies are introduced, revised, approved, and implemented within their state. The 22-question survey was developed to gather variables for the aims of the study. Descriptive statistics were calculated for each survey item. Of states who responded (n = 33), most reported a 2-committee (n = 24, 72.7%) process for developing and vetting policies, with initiation from the Sports Medicine Advisory Committee (n = 27, 81.8%), followed by an executive-level committee (n = 18, 66.7%). States reported total time from policy initiation to final approval ranged from 2 weeks to over 12 months. When a new policy was approved, most states indicated implementation began with an e-mail (n = 24, 72.7%) sent to Athletic Directors (n = 26, 78.8%). School principal or district superintendent were reported as the position in charge of compliance (36.4%, n = 12). Most SHSAAs use a 2-step process to write and review an athlete health and safety policy before approval. SHSAAs that require a longer policy development time could delay the implementation of important health measures. SHSAAs could consider additional communication methods to ensure information reaches all stakeholders.

Triceps surae shortening in children

BACKGROUND: The triceps surae is the main muscle that exerts propulsion power during walking and running. Its retraction changes the biomechanics of the foot, alters locomotion, and results in the secondary development of flatfoot deformity. Literature data on the clinical assessment of triceps surae retraction vary.
 AIM: To assess the threshold value of foot dorsiflexion in different clinical assessment methods of determining Achilles tendon shortening.
 MATERIALS AND METHODS: The study included the clinical assessment results of foot dorsiflexion in 167 patients (325 feet) with flatfeet aged 7–18 years. The foot dorsiflexion evaluation consisted of the assessment of isolated foot dorsiflexion and foot dorsiflexion with stabilization of the tarsal joints. To determine the involvement of the gastrosoleus complex in the pathological process, foot dorsiflexion was assessed with knee joint flexion and extension. The obtained data were subjected to correlation, regression, and Bland–Altman analyses.
 RESULTS: Strong correlations were found when foot dorsiflexion was assessed with the same knee joint position (assessment of isolated foot dorsiflexion and foot dorsiflexion with stabilized tarsal joints and knee joint flexion; the same tests with knee joint extension). Moderate correlations were noted when comparing foot dorsiflexion with knee joint extension and flexion. In the regression analysis, formulas were obtained according to which the threshold values of foot dorsiflexion were calculated in various variants during knee joint flexion and extension, indicating the retraction of the triceps surae: isolated foot dorsiflexion with knee joint extension 20°, isolated foot dorsiflexion with knee flexion 36°, and foot dorsiflexion with tarsal joint stabilization and knee extension 23°.
 CONCLUSIONS: Retraction of triceps surae in children with flatfeet equally involves the gastrocnemius and soleus. Differences in the magnitude of foot dorsiflexion with knee joint flexion and extension are the main factors in the retraction of the triceps surae to the gastrocnemius muscle.

Numerical simulation of the coupled dynamics model of the projectile engraving process

AbstractThe projectile engraving process directly influences the projectile motion in‐bore and impacts the firing accuracy, firing safety, and barrel life of the gun. For this reason, attention has been focused on this research topic. To address the limitations of the “instantaneous engraving” hypothesis adopted in the classical interior ballistic theory, the VUAMP user subroutine, one of ABAQUS's secondary development interfaces, is utilized in this paper to realize the modeling and numerical simulation of a coupled dynamics model of the projectile engraving process. In addition to facilitating engineering applications, a polynomial fitting formula of the engraving resistance obtained by simulation is proposed and then used as a supplement to establish a closed and solvable interior ballistic model considering the projectile engraving process. By comparing with test data, the simulation accuracy of the coupled dynamics model is verified. Simulation results reveal that the engraving process takes 3.8 ms, accounting for 26% of the whole launch process, which takes 14.6 ms, demonstrating that the process is not instantaneous. The results of this paper can serve as a reference for future studies on the coupled solution of the projectile engraving process and interior ballistics of guns or gun‐like equipment.

Milling characteristics of SiCp/2024Al composites thin-walled part based on movable auxiliary support

Due to milling force and milling heat, thin-walled parts are highly susceptible to deformation and even scrap. In this paper, a method of milling thin-walled part based on the movable auxiliary support was proposed. The ABAQUS software was employed to simulate the milling of thin-walled SiCp/2024Al composite part, while the movement of the auxiliary support was simulated using the secondary development subroutine VDLOAD. The influences of the magnitude and area of the movable auxiliary support on the surface temperature, deformation, and residual stress of the workpiece were investigated by single factor method. The results showed that, with the increasing of the magnitude and area of the movable auxiliary support, both surface temperature and deformation of the thin-walled part decrease. The tensile residual stress decreases and gradually changes to compressive residual stress. Interestingly, when the magnitude exceeds 20 MPa or the area exceeds 100 mm2, the deformation of the thin-walled part continues to decrease, but the middle of the thin-walled part is concave toward the milling cutter. It is worth noting that the effect of area on the residual stress does not show a clear regularity. Thus, the reasonable auxiliary support can reduce deformation and stability in the milling of thin-walled parts and the results of the study can provide a theoretical basis for optimizing the milling process of thin-walled parts.

A new method for predicting the oil displacement volume by waterflood huff-puff of the fractured-cavity type carbonate oil reservoirs

Introduction: More than two billion tons of oil reserves have been discovered in the fractured-cavity type carbonate reservoirs in Tarim Basin, which is a key field for increasing reserves and production in the future. This type of oil reservoirs is characterized by extremely high heterogeneity, and waterflood huff-puff oil displacement is generally used for secondary development of the oil reservoirs. The conventional methods are dominated by qualitative evaluation, which are insufficient to predict the oil production increase by waterflood huff-puff in various karst zones.Methods: The new method developed in this study involves four aspects, i.e., establishing a standard well database, optimally selecting the key development indicators, equivalent classification of reservoir space type, and optimally selecting the classification and evaluation methods for oil production increase by waterflood huff-puff. From the perspective of reservoir space type, the result of waterflood huff-puff oil displacement of multi-cave type reservoir (large scale) is better than that of relatively isolated cave type reservoir, followed by the fractured-vuggy type reservoir.Results and discussion: The new method shows advantages in the following aspects: 1) The differences in reservoir geological genesis and spatial structure characteristics in various karst zones are involved; 2) The difference in internal filling features of the same type of reservoir is integrally determined based on the changes in production performance; 3) The actual development characteristics of waterflood huff-puff oil displacement wells in various karst zones are systematically analyzed, and the criteria for selecting standard wells is formulated; 4) The violin plot is introduced as the main study tool for predicting and discriminating oil production increase by waterflood huff-puff displacement. Therefore, the new method is more capable of predicting the oil production increase by waterflood huff-puff in various karst zones.

Multiple roles of brassinosteroid signaling in vascular development.

Brassinosteroids (BRs) are plant steroid hormones that control growth and stress responses. In the context of development, BRs play diverse roles in controlling cell differentiation and tissue patterning. The vascular system, which is essential for transporting water and nutrients throughout the plant body, initially establishes a tissue pattern during primary development and then dramatically increases the number of vascular cells during secondary development. This complex developmental process is properly regulated by a network consisting of various hormonal signalling pathways. Genetic studies have revealed that mutants defective in BR biosynthesis or the BR signalling cascade exhibit a multifaceted vascular development phenotype. Furthermore, BR crosstalk with other plant hormones, including peptide hormones, coordinately regulates vascular development. Recently, the involvement of BR in vascular development, especially in xylem differentiation, has also been suggested in plant species other than the model plant Arabidopsis thaliana. In this review, we briefly summarize the recent findings on the roles of BR in primary and secondary vascular development in Arabidopsis and other species.

Long-Term Survival of a Child with Atypical Teratoid-Rhabdoid Tumor and Acute Lymphoblastic Leukemia: A Case Report

Atypical teratoid-rhabdoid tumor (AT/RT) is a rare but one of the most aggressive embryonal tumors of the central nervous system (CNS), most often occurring in children under 3 years of age. AT/RT accounts for about 1–2% of all CNS neoplasms and has a very poor prognosis, high risk of secondary tumor development, recurrence and/or metastasis in patients in remission and limited therapeutic potential. The clinical manifestations are usually symptoms of increased intracranial pressure. The mainstay of tumor treatment is complex chemotherapy combined with radiation therapy. A clinical case of sequential occurrence of two cancers (AT/RT and leukemia) in a 3-year-old girl is presented.

Simulation method for filling stage in liquid composite molding based on a two-phase flow volume of fluid model

Liquid composite molding (LCM) is a process for manufacturing fiber-reinforced resin matrix composites. The chemical rheological properties of resin in the filling stage of LCM will produce a non-isothermal flow process that includes multi-field coupling of thermal field, flow field, and chemical field. During the filling stage, the resin is injected into a closed mold pre-laid with a fiber at a certain temperature and pressure to flow continuously and undergo a curing chemical reaction. The viscosity of resin changes constantly under the influence of temperature and the curing reaction, which affects the flow of resin. The simulation of this multi-field coupling filling stage in LCM can provide an effective reference for the design of process parameters. The simulation method of the filling stage in LCM is studied in this paper. The mathematical model of the filling stage is derived based on the air–resin two-phase flow volume of the fluid model. Based on the secondary development of the ANSYS FLUENT software, the mathematical model is solved to realize the simulation. The parameters of an alkali-free glass fiber plain weave fabric are measured and the calculation model of thermosetting resin viscosity is established by designed experiments, which are applied in simulation. The filling stage experiment of LCM is designed to verify the accuracy of simulation results. Meanwhile, by comparing with the traditional simulation method, it is found that the data error obtained by the simulation method in this paper is significantly reduced.

Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production

Andrographis paniculata (Burm. f.) Nees is an important medicinal plant known for its bioactive compound andrographolide. NAC transcription factors (NAM, ATAF1/2, and CUC2) play a crucial role in secondary metabolite production, stress responses, and plant development through hormonal signaling. In this study, a putative partial transcript of three NAC family genes (ApNAC83, ApNAC21 22 and ApNAC02) was used to isolate full length genes using RACE. Bioinformatics analyses such as protein structure prediction, cis-acting regulatory elements, and gene ontology analysis were performed. Based on in silico predictions, the diterpenoid profiling of the plant’s leaves (five-week-old) and the real-time PCR-based expression analysis of isolated NAC genes under abscisic acid (ABA) treatment were performed. Additionally, the expression analysis of isolated NAC genes under MeJA treatment and transient expression in Nicotiana tabacum was performed. Full-length sequences of three members of the NAC transcription factor family, ApNAC83 (1102 bp), ApNAC21 22 (996 bp), and ApNAC02 (1011 bp), were isolated and subjected to the promoter and gene ontology analysis, which indicated their role in transcriptional regulation, DNA binding, ABA-activated signaling, and stress management. It was observed that ABA treatment leads to a higher accumulation of andrographolide and 14-deoxyandrographolide content, along with the upregulation of ApNAC02 (9.6-fold) and the downregulation of ApNAC83 and ApNAC21 22 in the leaves. With methyl jasmonate treatment, ApNAC21 22 expression decreased, while ApNAC02 increased (1.9-fold), with no significant change being observed in ApNAC83. The transient expression of the isolated NAC genes in a heterologous system (Nicotiana benthamiana) demonstrated their functional transcriptional activity, leading to the upregulation of the NtHMGR gene, which is related to the terpene pathway in tobacco. The expression analysis and heterologous expression of ApNAC21 22 and ApNAC02 indicated their role in andrographolide biosynthesis.

Methylation of microRNA genes and its effect on secondary xylem development of stem in poplar.

MicroRNAs (miRNAs) and DNA methylation are both vital regulators of gene expression. DNA methylation can affect the transcription of miRNAs, just like coding genes, through methylating the CpG islands in the gene regions of miRNAs. Although previous studies have shown that DNA methylation and miRNAs can each be involved in the process of wood formation, the relationship between the two has been relatively little studied in plant wood formation. Studies have shown that the second internode (IN2) (from top to bottom) of 3-month-old poplar trees can represent the primary stage of poplar stem development and IN8 can represent the secondary stage. There were also significant differences in DNA methylation patterns and miRNA expression patterns obtained from PS and SS. In this study, we first interactively analyzed methylation and miRNA sequencing data to identify 43 differentially expressed miRNAs regulated by differential methylation from the primary stage and secondary stage, which were found to be involved in multiple biological processes related to wood formation by enrichment analysis. In addition, six miRNA/target gene modules were finally identified as potentially involved in secondary xylem development of poplar stems through degradome sequencing and functional analysis. In conclusion, this study provides important reference information on the mechanism of interaction between different regulatory pathways of wood formation.

Application of Python-Based Abaqus Secondary Development in Laser Shock Forming of Aluminum Alloy 6082-T6

Aluminum alloy 6082-T6 is an important material for manufacturing the outer skin of high-speed trains, and laser shock forming can realize the rapid forming of complex-shaped plates. In order to improve the efficiency of the simulation modeling of laser shock forming for aluminum alloy 6082-T6, Python scripting language was used for the secondary development of Abaqus. A plugin was utilized to simulate and analyze the laser shock forming process of aluminum alloy 6082-T6. The coordinates of the plate after laser impact molding were measured using a coordinate measuring machine to calculate the arc bow height of the plate. The accuracy of the simulation model was verified by comparing with the simulation results. The deformation characteristics of plastic strain and arc height of aluminum alloy 6082-T6 under different laser process parameters were analyzed. The simulation plugin has a concise interface, high operability, and accurate results with the other parameters unchanged. When the laser energy is 5 J, 6 J, and 7 J, the corresponding arc heights are 5.9 mm, 6.6 mm, and 7.2 mm, respectively. As the thickness of the sheet increases, the deformation changes from concave at 1 mm to convex at 2 mm, 3 mm, 4 mm, and 5 mm. As the spot size increases from 1 mm to 5 mm, the transmission mode of the shock wave gradually changes from spherical wave to planar wave, and the arc height of the sheet increases from 4.6 mm to 8.2 mm. With the increase in the spot overlap rate, the impact area accumulates residual stress, and the arc height of the sheet is 5.7 mm, 6.6 mm, 7.3 mm, and 8.5 mm, respectively. The secondary development of ABAQUS 2021 using Python 3.6 scripting language has improved the efficiency of simulation modeling and provided reference for rapidly predicting the deformation characteristics of aluminum alloy 6082-T6 under different laser process parameters.

Parametric Design Method and Lift/Drag Characteristics Analysis for a Wide-Range, Wing-Morphing Glide Vehicle

The parametric design method is widely utilized in the preliminary design stage for hypersonic vehicles; it ensures the fast iteration of configuration, generation, and optimization. This study proposes a novel parametric method for a wide-range, wing-morphing glide vehicle. The whole configuration, including a waverider fuselage, a rotating wing, a blunt leading edge, rudders, etc., can be easily described using 27 key parameters. In contrast to the typical parametric method, the new method takes internal payloads into consideration during the shape optimization process. That is, the vehicle configuration can be flexibly adjusted depending on the internal payloads; these payloads may be of random amounts and have different shapes. The code for the new parametric design method is developed using the secondary development tools of UG (UG 10.0) commercial software. The lift and drag characteristics over a wide operational range (H = 6–25 km, M = 2.5–8.5, AOA = 0–10°) were numerically investigated, as was the influence of the retracting angle of the morphing wings. It was found that, for the mode of the fully deployed wings, the lift-to-drag ratio (L/D) remained at a high level (≥4.7) over a Mach range of 4.0–8.5 and an AOA range of 4–7°. For the mode of the fully retracted wings, the drag coefficient remained smaller than 0.02 over a Mach range of 4.0–8.5 and an AOA range of 0–5°. A wide L/D of 0.3–4.7 could be achieved by controlling the retracting angle of the wings, thus demonstrating a good potential for flight maneuverability. The flexible change in L/D proved to be a combined result of varying pressure distribution and edge-flow spillage. This will aid in the further optimization of lift/drag characteristics.

Psoriasis and Bullous Pemphigoid Developing Simultaneously During Systemic Administration with Bisoprolol and Losartan

Psoriasis and bullous pemphigoid are both chronic inflammatory skin disorders, involving abnormal immune responses. Their coexistence is often observed in the literature in the context of secondary development following an initial inflammatory process. In the majority of cases, the development of psoriasis seems to occur before the onset of bullous pemphigoid. While psoriasis is associated with autoimmune bullous diseases, it’s important to note that not every individual with psoriasis will develop bullous pemphigoid. Therefore the pathogenetic mechanism behind this association remains rather a mystery. Several case reports/studies have been published describing the subsequent development of either psoriasis or bullous pemphigoid following initial monotherapy with beta blockers, sartans, or other drug class medications (which will not be mentioned in our case report as they are not relevant to the presented data). We believe that our case is the first documented example of polymedicamentous development of 2 skin diseases within the intake of 2 antihypertensive drugs heterogeneous in class and action. We present a 78-year-old female with plaque psoriasis followed shortly thereafter by bullous pemphigoid after 10-year period of antihypertensive therapy with bisoprolol and losartan. We believe that the cumulative effect of both medications may have contributed to the coexistence of these skin disorders. Following the discontinuation of the initial antihypertensive therapy with bisoprolol and losartan and substituting it verapamil and moxonidine, along with the initiation of methotrexate and folic acid, the patient showed significant improvement. Key words: psoriasis; bullous pemphigoid; drug-induced; bisoprolol; losartan

Effect of dynamic constitutive differences in materials on the impact performance of CFST via secondary development of ABAQUS

To investigate the effect and adaptability of dynamic constitutive models on the impact performance of concrete-filled steel tubes (CFST), a numerical study on the dynamic properties of CFST under impact loading is conducted. By analyzing the impact response, including impact force, mid-span displacement, energy absorption, and mid-span bending moment, the effect of dynamic constitutive differences in materials on the impact performance of CFST is assessed quantitatively. Moreover, the HJC dynamic model was developed and validated by secondary development in ABAQUS. The findings show that the material model without considering strain rate effects would significantly underestimate the impact performance of CFST members. In contrast, the impact resistance of CFST is overestimated by using the J-C model and the C-S model with biased elastic conditions. The results obtained using the DIFavg model are most in agreement with the results of the actual model. The impact resistance of CFST is only slightly influenced by variations in the dynamic constitutive model of concrete. The impact performance of CFST is accurately predicted by using the VUMAT subroutine to program the HJC dynamic constitutive model.

Abstract 6373: Effectiveness of tumor surveillance across pediatric cancer predisposition syndromes

Abstract Background: Pediatric oncology patients are increasingly recognized as having an underlying cancer predisposing syndrome (CPS). For individuals diagnosed with a CPS, surveillance is often recommended to detect new tumors at their earliest and most curable stages. Screening is heavily based on radiologic imaging, however data on the effectiveness and outcomes of surveillance are limited. Methods: Patients with a clinical and/or molecular CPS diagnosis, including 274 children and young adults with 35 CPS at a single specialty pediatric oncology center, were monitored using standardized surveillance protocols over an average of 3 years (range 1 month - 12 years). Patients with hereditary retinoblastoma were excluded as surveillance was completed by a separate team. The cohort included 139 patients (50.7%) with a prior tumor history while 135 (49.3%) had no prior cancer. This study reviewed tumors diagnosed, method of detection, clinical course, and patient outcomes. Individual reports from 1,533 surveillance tests including diagnostic imaging, colonoscopy, and upper endoscopy, were assessed and classified for accuracy. Sensitivity, specificity, positive predictive value (PPV), and negative predictive values (NPV) were calculated for selected surveillance methods, and themes of common strengths or weaknesses of specific imaging modalities were identified. Results: During the study period from January 1, 2009 - September 31, 2021, 35 asymptomatic tumors were detected in 27 patients through surveillance (9.9% of the cohort), while five symptomatic tumors were detected in five patients (1.8%) outside of surveillance. Ten tumors (28.6%) discovered through surveillance were identified on first surveillance imaging. Among these 35 new tumors, 19 (54.3%) were diagnosed in children with a previously treated cancer, six (17.1%) were diagnosed in patients still being treated for cancer, and 10 (28.6%) were diagnosed in those with no prior cancer history. Malignant solid and brain tumors identified through surveillance were more often localized (20 of 24; 83.3%) than similar tumors detected before CPS diagnosis (71 of 125; 56.8%, p ≤0.0001). Of the 24 tumors identified through surveillance and surgically resected, 17 (70.8%) had completely negative margins. When analyzed across all CPS, the sensitivity (96.4%), specificity (99.6%), PPV (95.7%), and NPV (99.6%) of surveillance testing was high, with few false positive (n=6/1,533, 0.4%) or false negative (n=5/1,533, 0.3%) reports. No invasive interventions were performed due to false positive results. Conclusion: Patients with CPS are at increased risk of primary and secondary tumor development with 10% of our cohort developing a tumor in a short time span. Surveillance effectively enabled early detection of new brain and solid tumors across a wide spectrum of CPS which allowed for complete surgical resection and successful treatment in most cases. Citation Format: Melissa R. Perrino, Alise Blake, Cara E. Morin, Leslie Taylor, Rose B. McGee, Sara Lewis, Stacy Hines-Dowell, Arti Pandey, Paige Turner, Manish Kubal, Yin Su, Li Tang, Laura Howell, Lynn W. Harrison, Zachary Abramson, Ann Schechter, Noah D. Sabin, Kim E. Nichols. Effectiveness of tumor surveillance across pediatric cancer predisposition syndromes [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6373.

Research on Temperature Field of Cement-Mixing Pile-Reinforced Soft Soil Foundation

To investigate the mechanism of reinforcing soft soil with cement-mixing pile, based on ABAQUS secondary development, a numerical simulation study of the hydration reaction of cement-mixing piles was conducted. In this study, the influence of ground temperature variations on the distribution patterns of the temperature field in and around the pile was also considered. The temperature field of the pile–soil model can be primarily divided into two stages: the temperature rise stage (0~5 d) and the temperature decrease stage (5~90 d). The following observations were made: (1) The temperature of the pile body rapidly increased within the first 5 days, dissipating heat to the surrounding soil, leading to an elevation of the temperature in the soil around the pile and a decrease in soil moisture content. Around the 5th day, the temperature reached its maximum value, and the heat release rate of the pile body was higher than that of the surrounding soil. (2) With a 15% cement admixture, under the influence of 425# cement hydration, the temperature inside the pile increased by 5 °C, and the temperature in the soil around the pile increased by 4.2 °C. After considering the ground temperature, the temperature in the soil around the pile increased by 4.6 °C. (3) The maximum temperature generated during the hydration of 425# Portland cement is higher than that of 525#; the temperature of the soil around piles made with 425# cement is consistently higher than that made with 525#. (4) The hydration temperature of piles with a 10% cement admixture increased by 4.4 °C; for piles with a 15% cement admixture, the hydration temperature increased by 6.6 °C; and for piles with a 20% cement admixture, the hydration temperature increased by 9.1 °C. The temperature field of this structure gradually stabilizes after 7 days with increasing time and cement admixture. The results indicate that the hydration of cement-mixing piles raises the temperature of the soil around the piles. Additionally, the temperature resulting from the hydration of cement-mixing pile increases with the addition of cement.

Correlation between Structural and Functional Changes in Patients with Raised Intraocular Pressure Due to Graves' Orbitopathy.

This study explores the complication of secondary intraocular pressure (IOP) elevation and consequent glaucoma development in Graves' orbitopathy (GO), an autoimmune disorder associated with hyperthyroidism. Utilizing Octopus 900 visual field testing and optical coherence tomography (OCT), the research established correlations between functional and structural changes in optic nerve regions in patients with GO and patients with GO with elevated IOP (GO IOP) groups. A comparison with primary open-angle glaucoma (POAG) was conducted in a cohort of 182 subjects. The study identifies optic nerve head parameters that effectively differentiate changes in GO and GO IOP groups. In the GO group, the strongest correlation between structural and functional changes was observed in sector 7, while in the GO IOP group, it was in sectors 1 and 7. For POAG, correlation was found in six sectors. Elevated IOP in GO correlates with structural and functional impairments similarly to early glaucoma. Risk factors for GO-related elevated IOP included older age, longer duration of thyroid disease, and higher anti-thyroglobulin values. The study highlights the significance of regular IOP measurements, visual field assessments, and OCT examinations in GO patients. Early antiglaucoma intervention is warranted when characteristic structural and functional changes and/or risk factors are identified.

PagARGOS promotes low-lignin wood formation in poplar.

Wood formation, which occurs mainly through secondary xylem development, is important not only for supplying raw material for the 'ligno-chemical' industry but also for driving the storage of carbon. However, the complex mechanisms underlying the promotion of xylem formation remain to be elucidated. Here, we found that overexpression of Auxin-Regulated Gene involved in Organ Size (ARGOS) in hybrid poplar 84 K (Populus alba × Populus tremula var. glandulosa) enlarged organ size. In particular, PagARGOS promoted secondary growth of stems with increased xylem formation. To gain further insight into how PagARGOS regulates xylem development, we further carried out yeast two-hybrid screening and identified that the auxin transporter WALLS ARE THIN1 (WAT1) interacts with PagARGOS. Overexpression of PagARGOS up-regulated WAT1, activating a downstream auxin response promoting cambial cell division and xylem differentiation for wood formation. Moreover, overexpressing PagARGOS caused not only higher wood yield but also lower lignin content compared with wild-type controls. PagARGOS is therefore a potential candidate gene for engineering fast-growing and low-lignin trees with improved biomass production.

Mechanism interpretation of Guhan Yangshengjing for protection against Alzheimer’s disease by network pharmacology and molecular docking

Ethnopharmacological relevanceGuhan Yangshengjing (GHYSJ) is an effective prescription for delaying progression of Alzheimer’s disease (AD) based on the ancient Chinese medical classics excavated from Mawangdui Han Tomb. Comprising a combination of eleven traditional Chinese herbs, the precise protective mechanism through which GHYSJ acts on AD progression remains unclear and has significant implications for the development of new drugs to treat AD. Aim of the studyTo investigate the mechanism of GHYSJ in the treatment of AD through network pharmacology and validate the results through in vitro experiments. Materials and methodsChemical composition-target-pathway network and protein-protein interaction network were constructed by network pharmacology to predict the potential targets of GHYSJ for the treatment of AD. The interaction relationship between active ingredients and targets was verified by molecular docking and molecular force. Furthermore, the chemical constituents of GHYSJ were analyzed by LC-MS and HPLC, the effects of GHYSJ on animal tissues were analyzed by H&E staining. An Aβ-induced SH-SY5Y cellular model was established to validate the core pathways and targets predicted by network pharmacology and molecular docking. ResultsThe results of the network pharmacology analysis revealed a total of 155 bioactive compounds capable of crossing the blood-brain barrier and interacting with 677 targets, among which 293 targets specifically associated with AD, which mainly participated in and regulated the amyloid aggregation pathway and PI3K/Akt signaling pathway, thereby treating AD. In addition, molecular docking analysis revealed a robust binding affinity between the principal bioactive constituents of GHYSJ and crucial targets implicated in AD. Our findings were further substantiated by in vitro experiments, which demonstrated that Liquiritigenin and Ginsenosides Rh4, crucial constituents of GHYSJ, as well as GHYSJ pharmaceutic serum, exhibited a significant down-regulation of BACE1 expression in Aβ-induced damaged SH-SY5Y cells. This study provides valuable data and theoretical underpinning for the potential therapeutic application of GHYSJ in the treatment of AD and secondary development of GHYSJ prescription. ConclusionThrough network pharmacology, molecular docking, LC-MS, and cellular experiments, GHYSJ was initially confirmed to delay the progression of AD by regulating the expression of BACE1 in Amyloid aggregation pathway. Our observations provided valuable data and theoretical underpinning for the potential therapeutic application of GHYSJ in the treatment of AD.

Construction and application of online parametric modeling platform for face milling cutters based on WebGL and Open CASCADE

The secondary development of traditional commercial CAD/CAE software has been widely used in the process of cutting tools design and optimization. However, limited by copyright and hardware configuration, the effect of this development is limited, and the secondary development can no longer meet the needs of rapid and accurate cutting tools design. With the rapid development of Internet, online cutting tools CAD software based on Web has become a new development direction with its characteristics of specialization and lightweight. In order to build an online cutting tools CAD software, an online parametric design platform for indexable face milling cutters is established based on Web development technology, Open CASCADE and Three.js. The platform can quickly generate a 3D basic model for NC machining programing and a 3D detailed model for finite element simulation of cutting process according to ISO 13399. Based on the built platform, the finite element simulation model of cutting process of indexable surface milling cutter is established. The establishment of the model can play an important role in improving the design efficiency and reducing the design cost for the optimal design of this kind of milling cutter.